By Helmut Breuninger, Patrick Adam
There hasn’t been a publication relating "Microscopically managed surgical procedure" released and it is important to put up a ebook that information all of the varied phrases and technique utilized in microscopically managed surgical procedure. The aim is to create a pragmatic, concise and easy clarification of 3D-histology with workflows and designated illustrative fabric for dermatologists. it's for this reason designed to be a goal-oriented guide instead of an exhaustive reference paintings. it's going to give you the crucial info for all operating with sufferers present process this staff of treatments.
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Extra info for 3D Histology Evaluation of Dermatologic Surgery
Handling of Tumor Specimens of ca. 2 cm Diameter In the previous chapter, different kinds of excision and methods of flattening the borders of the margin were discussed independently. In the following examples of the procedure from excision, dividing the tissue and the conversion from 3D margins to a 2D plane for histology is shown. As discussed before the tumor can either be removed together with the safety margins in one step and divided later or the clinical 50 Chapter 4. Dividing Tumors and Topographical Orientation visible tumor and the margins are removed in consecutive excisions.
Cryo-sectioning The tissue that has been embedded in cryogel and attached to a tissue holder is now placed into a cryostat and sectioned (Fig. 9a, b). 5 nm, the tissue is taken up on a glass slide by pressing the front side of the slide onto the still frozen tissue (Fig. 9c). 9 Cryo-sections are taken from their front side on the front side of the slide which will be then flipped over should now represent the complete outside of the previously bowl-shaped specimen. The glass slide with the attached tissue is now flipped over to its front side (Fig.
As before, all important procedures and steps are illustrated to help the reader. Paraffin Sectioning In this section, the preparation and sectioning of the tissue using paraffin embedding will be described. For paraffin sectioning, a prior formalin fixation of the samples is required. Usually the 30 Chapter 3. Embedding and Sectioning excised tissue is fixed immediately after excision and 3D histology can be performed on the prefixed material. , not-fixed material is smoother and therefore easier to flatten.
3D Histology Evaluation of Dermatologic Surgery by Helmut Breuninger, Patrick Adam