By Frank J. Dixon, K. Frank Austen, Leroy E. Hood, Jonathan W. Uhr (Eds.)
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Additional info for Advances in Immunology, Vol. 40
The inclusion of either r-IL-2 or r-IFN-y instead of T cell supernatant during the initial activation with SA also resulted in enhanced subsequent responsiveness to either T cell supernatant or r-IL-2. 8 B cells were preincubated as described in the legend to Table X in the presence of various combinations of SA, T cell supernatant, 100 U/ml r-IL-2, or 100 U/ml r-IFN-y. ISC were quantitated on day 3 of the second incubation. Adapted from Jelinek et al. (1986b). 44 DIANE F. JELINEK AND PETER E. LIPSKY was somewhat less active than r-IL-2.
1983). Muraguchi and co-workers (1982) demonstrated BCGF in the MI 20,000-30,000 fraction and were able to separate this activity from IL-2. , 1983; Ambrus and Fauci, 1985). Shimizu et al. , 1985; Kishimoto, 1985). It is important to note that the high-molecular-weight human BCGF also supported differentiation. Therefore, the separation of the two BCGFs into types I and I1 provide a convenient means to distinguish the high- and low-molecularweight forms. However, a precise functional discrimination awaits further study.
Mond and colleagues (1985a) showed that r-IFN-y inhibited the murine B cell proliferative response stimulated by soluble anti-Ig antibody and could also partially suppress the induced expression of Ia. , 1985; Bich-Thuy and Fauci, 1986). Results from both of these studies also indicated that in contrast to IL-2, IFN-y alone did not support B cell growth. , 1985). , 1985). 38 DIANE F. JELINEK AND PETER E. LIPSKY Considerable controversy remains concerning the activation status of B cells responsive to IFN-y.
Advances in Immunology, Vol. 40 by Frank J. Dixon, K. Frank Austen, Leroy E. Hood, Jonathan W. Uhr (Eds.)