By David W. Murhammer (auth.), David W. Murhammer (eds.)
Baculovirus Expression Protocols is an in depth consultant for utilizing the baculovirus expression vector method (BEVS) and/or insect cells to supply recombinant proteins. techniques for the advance and construction of baculovirus pesticides at either laboratory and massive scale are defined within the form of step by step structure that the equipment in Molecular Biology™ sequence helped standardize. This publication is a one-stop resource for info on baculoviruses, and comprises sections on utilizing changed baculoviruses to specific genes in mammalian cells, utilizing Drosophila telephone traces, setting apart new cellphone strains, and constructing serum-free medium. particular purposes, together with utilizing baculovirus and bug phone method to review apoptosis, also are mentioned. This up-to-date and improved variation of Baculovirus Expression Protocols offers an ideal lab handbook for biochemists, molecular biologists, biochemical engineers, and others for utilizing the BEVS or insect cellphone strains to provide recombinant proteins.
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Extra resources for Baculovirus and Insect Cell Expression Protocols
Blissard, G. , and Rohrmann, G. F. (1989) Location, sequence, transcriptional mapping, and temporal expression of the gp64 envelope glycoprotein of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus. Virology 170, 537– 555. Pearson, M. , and Rohrmann, G. F. (2000) Identification of the Lymantria dispar nucleopolyhedrovirus envelope fusion protein provides evidence for a phylogenetic division of the Baculoviridae. J. Virol. 74, 6126–6131. Pearson, M. , Russell, Rebecca L. , and Rohrmann, G.
Oligomerization of IE-1 is thought to occur in the cytoplasm, before localization to the nucleus, binding to hr sequences, and subsequent enhancement of promoter activity through interaction with components of the basal transcription complex (83,85). 3. Transactivational Regulators A number of transactivational regulators of baculovirus early gene promoters havenumber of transactivational regulators of baculovirus early gene promoters have been identified. The immediate-early gene, ie-1, is thought to be the principal transregulator of early baculovirus expression and was originally identified due to its trans-acting regulatory role in 39K expression (80).
Baculovirus Choristoneura fumiferana multicapsid nuclear polyhedrosis virus p10 gene indicates that the polypeptide contains a coiled-coil domain. J. Gen. Virol. 76, 2923–2932. 40 Horton, H. M. and Burand, J. P. (1993) Saturable attachment sites for polyhedron40. derived baculovirus on insect cells and evidence for entry via direct membrane fusion. J. Virol. 67, 1860–1868. 41 Flipsen, J. T. , Martens, J. W. , van Oers, M. , Vlak, J. , and van Lent, J. 41. W. M. (1995) Passage of Autographa californica nuclear polyhedrosis virus through the midgut epithelium of Spodoptera exigua larvae.
Baculovirus and Insect Cell Expression Protocols by David W. Murhammer (auth.), David W. Murhammer (eds.)